Russian President Vladimir Putin denounced the EU's "confrontational" stance during a phone call with European Council President Charles Michel on Monday, ahead of a summit of EU leaders.
"Vladimir Putin gave an appraisal of the unsatisfactory state of Russia-EU ties which has emerged due to unconstructive, often confrontational policies of our partners," the Kremlin said in a strongly-worded statement.
Putin stressed that Russia was ready to "resume normal depoliticised" ties with the European Union if there's a will to do so in Brussels, the Kremlin said.
EU leaders meet for a summit in Brussels on Thursday and Friday.
Putin and Michel also discussed the fight against the coronavirus and potential use of Russia's homegrown vaccine Sputnik V as well as the situations in Ukraine and Belarus, the Kremlin statement said.
The call came after a top EU executive, Thierry Breton, said the European Union had no need for Sputnik V in remarks that Moscow said were "clearly biased".
The Kremlin is also facing a crisis in ties with the United States, with President Joe Biden describing Putin as a "killer" and rejecting his offer to hold public talks.
Moscow said Monday it regretted that Washington did not respond to Putin's proposal to organise public talks with the US leader.